Journal: Journal of Nanobiotechnology

Article Title: The biodistribution of self-assembling protein nanoparticles shows they are promising vaccine platforms

doi: 10.1186/1477-3155-11-36

Figure Lengend Snippet: Peptide sequences used. Green = pentamer, Blue = trimer, Red = bombesin, Black = linkers and His-tag, Orange = amino groups used for PEGylation.

Article Snippet: The PEGylation agent mPEG-NHS (1 kDa) was purchased from Nanocs, Inc., New York, USA.

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: The biodistribution of self-assembling protein nanoparticles shows they are promising vaccine platforms

doi: 10.1186/1477-3155-11-36

Figure Lengend Snippet: SDS-PAGE analysis of the PEGylation products. Left, the P6c peptide: A) Mw Marker B) uncoupled C) coupled with PEG; Right, the P6c-PbK peptide: D) Mw Marker E) uncoupled; F) coupled with PEG. For both constructs also the trimer band is visible on the gel.

Article Snippet: The PEGylation agent mPEG-NHS (1 kDa) was purchased from Nanocs, Inc., New York, USA.

Techniques: SDS Page, Marker, Construct

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Tumor Cell Distinguishable Nanomedicine Integrating Chemotherapeutic Sensitization and Protection

doi: 10.3389/fbioe.2021.773021

Figure Lengend Snippet: Characterization of COPIRS-DA-PEG. (A) The synthetic route of COPIRS-DA-PEG. n≈41; (B) FT-IR spectra of mPEG-Mal, mPEG-DA, COPIRS, and COPIRS-DA-PEG; (C) 1 H-NMR spectrum of mPEG-DA; (D) 1 H-NMR spectrum of COPIRS-DA-PEG; (E) 1 H-NMR spectrum of COPIRS-DA-PEG after incubation at 65°C and dialysis.

Article Snippet: Maleimide-functionalized PEG (mPEG-Mal) (Mw ≈ 2 kDa) and t-Boc-NH-PEG-Mal (Mw ≈ 2 kDa) were purchased from JenKem Technology Co. Ltd (Beijing, China).

Techniques: Incubation